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What should I pay attention to when operating the biosafety cabinet?

Technical Knowledge 2019-04-04 15:49:52
The biological safety cabinet is an air purification equipment that provides a local dust-free and sterile working environment, and can artificially control the discharged air in the working area through a special filtering channel to avoid harm to people and the environment. Safe microbiological dedicated clean bench. It can also be applied to related industries such as biological laboratory, medical and health, bio-pharmaceutical, etc., and has good effects on improving process conditions and protecting the health of operators.

Pay attention to the following items when using the biosafety cabinet daily:

1. When using the biosafety cabinet, first open the isolation window with the remote control, and the form alarm will sound. When the form slides up to the appropriate working height, the alarm sound is released and you can start working.

2. Do not put any items unrelated to this experiment in the biosafety cabinet. All the items required for the experiment should be moved into the safety cabinet before operation. (Working paper, mouse padding and other easily blown items are not allowed to be placed in the biological safety cabinet.) In order to avoid blocking the return air damper, avoid frequent interruption of airflow through the air curtain during operation; and disinfect the surface with 70% alcohol before disinfecting to disinfect the work surface. The items in the cabinet should be placed in a clean area, a semi-polluted area and a contaminated area. The items placed in the cabinet should be placed horizontally as far as possible. The items in the operation are convenient to use and avoid cross-contamination during the return air process. . Items should be placed as far back as possible, but the airway opening should not be blocked to avoid interference with normal airflow.

3. Turn on the fan for 5 to 10 minutes, and then perform the experimental operation after the air in the cabinet is cleaned and the airflow is stable. The arms should be slowly extended into the safety cabinet vertically, at least for 1 minute, to stabilize the airflow in the cabinet, and the hand should be moved as gently as possible during the operation of the cabinet. If you really need to take out your arm or item during the operation, you should also slowly enter and exit vertically, and you may be exposed to infected items. The gloves and arms should be surface-sterilized.

4. Avoid vibration principle: Try to avoid the use of vibration instruments (such as centrifuges, vortex oscillators, etc.) in the cabinet, because the vibration will cause the particulate matter accumulated on the filter membrane to shake off, resulting in a decrease in the cleanliness inside the operating room. Failure to balance the front operating surface can also cause contamination of the operator by the safety cabinet. If necessary, place it near 1/3 of the back wall of the cabinet. Clean items should be placed at least 150 mm away from the aerosol generating container.

5. Principle of movement in different sample cabinets: Operate and move items in accordance with cleaning areas (low-pollution items) to contaminated areas (highly polluting items) to avoid cross-contamination. To prevent possible spills, a towel or gauze soaked in a disinfectant can be placed on the countertop, but the safety cabinet grille should not be covered.

6. During the operation of the biosafety cabinet, it is strictly forbidden to use an open flame such as an alcohol lamp to avoid the heat flow caused by the airflow in the cabinet. The fine particle impurities generated during the open flame process will be brought into the filter area. Damage to the HEPA filter. When it is unavoidable that it is necessary to use it, it is advisable to use a Bunsen burner with a low flame. Micro electric heaters or infrared sterilizers are permitted, preferably disposable sterile inoculating rings;

7. Minimize the movement of people behind the work and quickly switch the door to prevent unstable airflow in the safety cabinet.

8. During the experimental operation, the glass window should not be opened, and the operator's face should be above the working window. Operate as far as possible in the center of the workbench. The diluted virus sample must be in the biosafety cabinet. It should not be blown and mixed on the vortex mixer. The aerosol generated by the blow is very likely to cause pollution in the whole test environment; the test tube containing the sample or test The surface of the disc is sealed to prevent the downdraft from invading the sample.

9. Biosafety cabinets should be tested and maintained regularly to ensure proper operation. If the safety cabinet is found to be working abnormally during work (such as continuous airflow alarm, indicating that the normal airflow mode of the safety cabinet is disturbed, the operator or the item is immediately in danger), stop working immediately, take corresponding measures, and notify the P2 administrator. .

10. Allow 24 hours a day to work. Continuous work helps control the level of dust and particles in the laboratory; it is recommended to keep the cabinet operating at all times. After the work is completed, close the glass window, keep the fan running for 10 to 15 minutes, and turn on the UV lamp for 30 minutes.

11. The contaminated items to be discarded should be temporarily placed at the rear of the safety cabinet; avoid placing the items to be discarded in the container outside the safety cabinet during operation. Larger items, such as waste bags, plates containing discarded straws, and suction filter bottles, should not be taken out of the safety cabinet on one side; all items that need to be discarded should be placed in yellow biosafety bags or waste containers. Inside, the seal can be taken out after the seal is tight; the items used in the cabinet should be removed after the surface is disinfected to prevent the pathogenic microorganisms from being taken out and polluting the environment.

12. The biosafety cabinet should be cleaned and disinfected regularly. The contaminants in the cabinet can be wiped with 2% 84 disinfectant after the work is completed and the UV lamp is disinfected. 70% alcohol can also be used to disinfect the work surface, rear wall, side wall, and the inside of the observation window; the outer surface of the cabinet should be wiped with 1% 84 disinfectant per day. Formaldehyde steam fumigation is the most common method of removing pollution.

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